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what is the best way to recover a soluble protein that I cloned into E. coli that forms inclusion bodies therefore losin
Posted by People • 8/16/09 • Subscribe to this Discussion [RSS] • Report This Topic
Topics: soluble protein
what is the best way to recover a soluble protein that I cloned into E. coli that forms inclusion bodies therefore losing all activity and making it almost impossible to purify?
Just wondering.
User Comments
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Just wondering, huh? -
Just wondering or you are on your way finishing your assignments?
Okay I am now searching around.-
Apparently, there are so much answers from the google search result for the search term of "what is the best way to recover a soluble protein that I cloned into E. coli that forms inclusion bodies therefore losin"
see it here by yourself: tiny.cc/i2Z2R
Always remember this method. When you are about blind, no clue at all or just being extra lazy, type in the whole question no matter how long and there should be result(s) appears.
for the answer: I choose this long article: What Is Prokaryote? www.bionewsonline.com/6/what_is_prokaryote.htm (just find it yourself: the answer is there)
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I've often wondered that myself. -
You should at least gave the credit for who ask that question. That was me and this is plagiarism.-
Are you seriously using the P word? I was just using your joke here as a reference to the other thread. You look so happy on your avatar and now you accuse me of stealing your internet?
So for anyone who's interested, Deray28 used this line in a comment on a different thread. There.
I thought you'd be happy to see that, honestly.
My lawyers have been informed. -
I'm sorry if I over reacted People. I have no intentions of making this a legal matter. But, it is not on to take ideas like that and run with them. You could have asked first.
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dehydration -
Follow Alice through the rabbit hole.-
that is probably the only thing I haven't tried! jajajajaa
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